Quantum Dots-Based Immunochromatographic Strip for Rapid and Sensitive Detection of Acetamiprid in Agricultural Products

Quantum Dots-Based Immunochromatographic Strip for Rapid and Sensitive Detection of Acetamiprid in Agricultural Products

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In this study, a rapid and sensitive immunochromatographic strip (ICS) assay, based on quantum dots (QDs), was developed for the qualitative and quantitative detection of acetamiprid in agricultural samples.Acetamiprid-ovalbumin conjugates (ACE-OVA) and goat anti-mouse IgG were sprayed onto a nitrocellulose membrane as a voyage et cie discount code test and control line.Two kinds of anti-acetamiprid monoclonal antibodies (mAb) obtained in our lab were characterized by the ELISA and surface plasmon resonance assay.

The competitive immunoassay was established using a QDs-mAb conjugate probe.The visual detection limit of acetamiprid for a qualitative threshold was set as 1 ng/mL to the naked eye.In the quantitative test, the fluorescence intensity was measured by a portable strip reader and a standard curve was obtained with a linear range from 0.

098 to 25 ng/mL, and the half maximal inhibitory concentration of 1.12 ng/mL.The developed method showed no evident cross-reactivities with other neonicotinoid insecticides except for thiacloprid (36.

68%).The accuracy and precision of the developed QDs-ICS were further evaluated.Results showed that the average recoveries ranged from 78.

38 to 126.97% in agricultural samples.Moreover, to test blind tea samples, the QDs-ICS showed comparable reliability and a high correlation with ultra-performance liquid chromatography-tandem mass spectrometry.

The whole sample detection could be accomplished within 1 h.In brief, our data clearly manifested that QDs-ICS was quite qualified for the rapid nyx 22 brush and sensitive screening of acetamiprid residues in an agricultural product analysis and paves the way to point-of-care testing for other analytes.